Portsmouth, with debts of about £60m, have become the first Premier League club to enter administration.
The club will also be docked nine points as a result which means relegation is almost certain.
Pompey were due to face a winding-up order on 1 March but decided to go into administration unless a buyer for the club was found.
It was reported that four parties were considering a takeover but Thursday's deadline passed without a conclusion.
Administrator Andrew Andronikou, of insolvency experts UHY Hacker Young, now has the responsibility of beginning the process of cutting costs at the club to try to keep it as a viable entity.
It follows weeks of speculation over the survival of the cash-strapped south coast side and is a stark warning for all football clubs.
Portsmouth employ nearly 600 people - directly or indirectly - and accountant Nick O'Reilly of Vantis, who recently examined the club's books, said the club was "completely dysfunctional" and warned them to expect a "rocky" time ahead.
"The next few months are crucial to the business," he said. "People will lose jobs, but hopefully the club will come out the other side."
As well as struggling at the bottom of the Premier League, currently seven points behind second-placed Burnley, Portsmouth have suffered a catalogue of ongoing financial woe.
Players have been paid late on four occasions this season, while the club is also involved in a separate dispute with former owner Sacha Gaydamak over whether they have missed a deadline in paying a £9m chunk of the £28m they owe him.
The Premier League also withheld £2m of transfer payments and diverted a £7m slice of TV revenue to Chelsea and Watford to cover the signings of Glen Johnson and Tommy Smith.
They are also being sued by former defender Sol Campbell for £1.7m for unpaid image rights.
Despite their precarious financial position, O'Reilly believes the club will continue to exist, while former Pompey boss Paul Hart, sacked in November after just four months in charge, thinks administration could provide a platform for a "fresh start".
He told BBC Radio 5 live: "I think the club can be strong again if they use some foresight and planning and adopt a restructuring programme.
"It looks like administration is necessary and hopefully will give the club a chance to recover."
Hart added: "The supporters have been long suffering and there are some very good, conscientious people who work there we should be thinking about because their jobs are in a precarious position."
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